Lobna, A. Moussa and Abd El Monem Mobarak Daoud( 2013). Steel slag by-product with microbacterium trichotecenolyticum as biofertilizer in corn (zea mays l) in EgyptJ. Biol. Chem. Environ. Sci., 2013, Vol. 8(2):373-400
Abstract: Domestic isolate of Microbacterium trichotecenolyticum was successfully identified by 16Sr-RNA gene sequence analysis using universal primer. The biolog identification method failed to resolve speciation of these bacteria. In vitro studies, the tested bacterium was found capable of releasing Silicon (max 92 µg SiO2/ml) and potassium (max 105 µg K/ml) from both steel slag as an iron industry by-product. Similar trend with mica, as a source found in different types of rocks, was also documented. In vivo studies with Corn, Zea mays, planted in a well characterized loamy sand soil amended with steel slag, bacterization with M. trichotecenolyticum was tried. Two soil investation levels of the bacterium versus two amendment rates of steel slag were experimented. The results revealed that the combined treatment (s) gave higher grain yield and greater NPK content compared to either single bacterial treatment or single slag ones. The combined application of steel slag at a rate equivalent to 250 kg/fed and M. trichotecenolyticum at 5 L broth/fed increased grain and straw yields by 42.6 and 33.9 %, respectively, compared to the single slag rate. The same application rate increased the grain potassium and phosphorous content as well. The results also indicated remarkable effect of 500 kg /fed and 10L broth/fed treatment on increasing nutritional status of Si, P and K by 115, 15.7 and 47 ug/g, respectively, with non significant effect on heavy metals build up in soil. The maximum grain yield was achieved with 5L of M. trichotecenolyticum and 250 Kg steel slag / fed.
Keywords: (Microbacterium trichotecenolyticum, steel slag, mica, corn heavy metals, silicon, nutrients elements.)
