Lobna A. Moussa and Ahmed E. Mohy( 2013). Optimization of growth conditions for proper agrocin production by rhizobium radiobacterN. Egypt. J. Microbiol. Vol. 34, January, 2013.
Abstract: A chemically defined medium was developed to support the growth of domestic isolate of Rhizobium radiobacter (HQ 395610-Egypt) and its production of agrocin. Various supplements were investigated for their effect on production of agrocin 84 using the method of well-diffusion assay against Agrobacterium tumefaciens. D-fructose as carbon source was found to be a better substrate for agrocin production compared to the other sugars and sugar alcohols tested, while monobasic ammonium phosphate was the best nitrogen source than other organic and inorganic ones. On the other hand, scaling up C/N ratio to 1:16 under pH 5.5 at 35˚C incubation temperature maximized the production after 12 hr. In the fermentor, scaling up to 1L maximized the results nearly twice at agitation speed of 200 rpm and airflow of 1 Lpm. Agrocin fragments were successfully separated, partially purified and well located by UV spectrum at 254 nm.
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