Gehan H. Abd El-Aziz, Shreen S. Ahmed, K. El Mangoury and Ashraf H. Fahmy( 2016). Using Different Growth Regulators in Wheat to Overcome Negative Effects of Drought Stress as One of Climate Change Impacts and Evaluation of Genetic Variation Using ISSRAdvances in Environmental Biology, 10(6) June 2016, Pages: 82-91
Abstract: Drought is a worldwide problem, constraining global crop production seriously and recent global climate change has made this situation more serious. This study was planned to evaluate the potential of kinetin and salicylic acid as a growth regulator to overcome the negative effects of stimulated drought stress on two wheat (Triticum aestivum L.) cultivars (Gimeza 9 and Sakha 93). Two experiments (pots and field) were carried out during two successive seasons. Water deficit was imposed by withholding water after milking stage to season end. Yield and quality traits were evaluated at end time. Results showed that drought stress caused an increase in proline, total carbohydrates, catalase and peroxidase enzyme while decrease grain yield, protein (gluten) and macronutrient content (N, P, and P) of both wheat cultivars. The impact of drought stress is more damaging in the Gimeza 9 cultivar compared with Sakha 93 cultivar. Kinetin and salicylic acid treatments reduced the damaging action of drought stress on wheat plants. Both kinetin and salicylic acid treatments increased wheat grain yield, biochemical characters and antioxidant enzyme content under control and stress conditions. These effects were more pronounced with kinetin treatment. The impact of kinetin and salicylic acid used to overcome drought damage was more pronounced on the Sakha 93 cultivar than Gemiza 9 cultivar . Moreover, using ISSR was robust and detects minor variations fast, reliable and efficiently for both tested wheat cultivars treated to adopt drought stress.
Keywords: (Stress, Wheat, Stress tolerance, Growth regulators, Kinetin, Salicylic acid, Antioxidant enzymes, Proline; ISSR.)