Afifi, M.M.; Massoud , O. N. and El- Akasher, Y. S.( 2015). Bioethanol Production By Simultaneous Saccharification And Fermentation Using Pretreated Rice Straw Middle East Journal of Applied Sciences, 6(4): 1022-1034, 2015
Abstract: The shopped rice straw was pretreated under different concentrations of both acid (sulfuric acid) and alkaline (sodium hydroxide) at room temperature and heat under pressure at 121 C0 for 15 min. The optimum pretreatment was carried out with 4% NaOH and 2% H2SO4. The effective of alkaline pretreatment is more in delignification than acid pretreatment. Six microbial strains isolated from compost piles located at Training Center for Recycling of Agricultural Residues (TCRAR) were tested on basal salt medium using pretreated rice straw as a sole carbon source. Results obtained that both fungal species Aspergillus niger and Trichoderma viride were the most efficient strains to detect more cellulases and A. niger is considered more efficient than T. viride. Where it recorded 3.5 µ mol/ ml / hr. By using A. niger during saccharification process, the release of more total sugars is induced by alkaline pretreatment whereas acid pretreatment attained more reducing sugars. The highest values of total and reducing sugars were recorded after 24 h. The consumption of glucose was lower during first 18 h of fermentation by Saccharomyces cerevisiae. However, the highest percentage of ethanol 5.5% (v/v) produced after 96 h.
Keywords: (pretreatment, rice straw, saccharification, fermentation, A. niger, T. viride, S. cerevisiae, bioethanol.)
