Soad Y.S. El-Sayed, Hend, M. El-Egami and Yasser A. El-Tahlawy( 2016). Symbiotic n2-fixation efficiency of lupine plant under fungal and bacterial inoculationEgypt. J. Biotechnol. Vol. 52, June, 2016.
Abstract: Plant growth promoting fungi (PGPF) and Plant growth promoting bacteria (PGPB) represent the highest portion of microorganisms (PGP-microorganisms) that inhabit the rhizosphere (PGP-Microorganisms). So, Trichoderma harzianum and Serratia marscences and Bradyrhizobium sp. were examined in vitro for achieving the traits that being related to plant growth promoting effects such as, indol acetic acid (IAA) phytohormome production and phosphate solubilization. Furthermore, a field experiment was conducted to study the co-inoculation effect of T. harzianum, S. marscences and their mixture on the symbiotic N2-Fixation, some plant growth char-acters and yield of lupine plant. The in vitro, all tested microorganisms were appar-ently able to trigger PGP-properties. However, T. harzianum appeared to be superi-or in P-solubilization and IAA production regarding to the other tested microorgan-isms. The available phosphorus measured was (104.61, 96.72 and 83.72 ppm) for T. harzianum, S. marscences and Bradyrhizobium, respectively. While the amounts of IAA produced were (105.84, 79.03 and 60.5 μg ml-1) by T. harzianum, Bradyrhizobi-um and S. marscences, respectively. At the vegetative stage, the results of field trail displayed that the co-inoculation of Bradyrhizobium with T. harzianum, S. marscences and their mixture had significant effect on nodulation status (nodule number, nodule dry weight and nitrogenase activity) comparing to control treatment of sole Bradyrhizobium inoculation. However, the treatment of T. harzianum was surpassed the other treatments. The same trend was recorded at the harvest stage, where T. harzianum conducted with Bradyrhizobium, which exhibited superiority for approve the lupine yield and its components (biological and seed yield, 100 seed weight, harvest index and seed crude protein).
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