Zeinat K.Mohamed, MohamedA.Ahmed, NashwaA.Fetyan, Sherif M.Elnagdy( 2010). Isolation and molecular characterisation of malathion-degrading bacterial strains from waste water in EgyptJournal ofAdvancedResearch(2010) 1, 145–149
Abstract: Efficienciesoflocalbacterialisolatesinmalathiondegradationwereinvestigated.Fivebacterial isolates btainedfromagriculturalwastewaterwereselectedduetotheirabilitytogrowinminimalsalt media, uppliedwith250ppmmalathionassolesourceofcarbonandphosphorus.Thepurifiedbacterial isolates (MOS-1,MOS-2,MOS-3,MOS-4andMOS-5)werecharacterisedandidentifiedusingacom- bination ofcellularprofile(SDS-PAGE),geneticmakeupprofile(RAPD-PCR),andmorphologicaland biochemical characteristics.Fourbacterialisolates(MOS-1,MOS-2,MOS-3andMOS-4)withidentical genetic characteristicswereidentifiedas Enterobacteraerogenes, whereasisolateMOS-5wasidentifiedas Bacillus thuringiensis. Thedegradationrateofmalathioninliquidculturewasestimatedduring15days of incubationfortheisolateMOS-5of B. thuringiensis. Slightlymorethan50%oftheinitialmalathion wasdecomposedwithin3days.Themalathionconcentrationdecreasedtoalmost17%intheinoculated medium after10daysincubation,whilemorethan91%oftheinitialmalathionwasdegradedafter15days.
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