Ghada W. Abou-El-Souod, Ebtsam M. Mors y and Lamiaa H. S. Hassan( 2017). Production of Bioethanol from microalga (Chlorel la vulgaris)Egypt. J. Exp. Biol. (Bot.), 13(1): 49 – 54 (2017) The Egyptian Society of Experimental Biology
Abstract: Bioethanol production from algae requires four major unit operations including: pretreatment, hydrolysis, fermentation and distillation. Because Chlorel la vulgar is biomass has a soft thallus structure and high moisture content. The biological processes have been used for pretreatment of algal mater ials. The Chlorella vulgaris biomass was subjected to saccharification process by the fungal organism Aspergillus niger for the hydrolysis, this process was fol lowed by the fermentation using yeast Saccharomyces cerevisiae to produce alcohol. The biological hydrolysis pretreatment was done on two biomass samples. The fi rst sample was treated with 1% NaOH for one hr. before treated with Aspergilus niger. While the second biomass sample was untreated with 1% NaOH. High sugars was released on the 6th day of saccharification in the case of untreated sample with NaOH. High contents of reducing sugars were obtained in the biomass of Chlorella vulgar is under the condition of the synthetic media by Aspergillus niger at the 6th day of experiment 8.552 (g /100 g dried biomass) and 19.835 (g /100 g dried biomass). Consequently, bioethanol increased at the synthetic media releasing the maximum amount of 3.68 (g /100 g dried biomass) at the 1s t day which increased gradually till reaching 25.20 (g /100 g dried biomass) at the 5th day. Maximum amount of reducing sugars was obtained at the 1st day 6.996 (g /100 g dried biomass) which increased till reaching 16.052 (g /100 g dried biomass) at the 6th day in the case of treated samples with 1% NaOH. Consequently, the maximum amount of bioethanol was produced at the 1st day 10.95 (g /100 g dried biomass) which increased gradually till reaching 16.77 (g /100 g dried biomass) at the 4th day
Keywords: (Bioethanol , microalgae, Chlorella vulgaris, Biological method, Aspergillus niger ,)
