Sanaa S.H. Sarabana, Khadiga I. M. El-Gabry and Ahmed M. Eldin( 2015). Optimizing Growth Conditions Provoked Ethanol Production by Fungi Grown on GlucoseMiddle East Journal of Applied Sciences (2015) Volume 5 (4): 1222-1231
Abstract: This study was conducted to test nine cellulytic filamentous fungi including: Aspergillus oryzae, Aspergillus versicolor-I, Aspergillus versicolor-II, Fusarium oxysporum, Fusarium oxysporum-I, Mucor indicus, Penicillium citrinum, Phanerochaete chrysosporium and Pleurotus sp., to ferment glucose. They were tested on Mandels medium during 14 days of incubation at 35°C under minor air conditions. The best ethanol producers were F. oxysporum, Ph. chrysosporium and F. oxysporum-I, recording 0.464, 0.360 and 0.325g ethanol/g carbon. Modifications to Mandels medium were done individually to magnify ethanol production and deselect aside lower ethanol producers. Nitrogen sources substitution including ammonium sulfate, ammonium phosphate, casein, peptone, potassium nitrate and yeast extract was done. Different C:N ratios (2:1, 4:1, 6:1 and 8:1) and pH levels (4, 5 and 6) were tested. Glucose concentrations including 100, 200 and 300g/L were also tested. Ph. chrysosporium was selected for its best production recording 0.847 g ethanol/g carbon corresponding to 33.9 g/L, utilizing yeast extract as N source, at C:N ratio of 2:1, pH=5 and initial glucose level of 100g/L. The ethanol yield was improved 2.4 times (from 14% to 33.9%). However, either pH=4 or 300g/L glucose was repressor for ethanol production.
Keywords: (Growth Conditions Provoked Ethanol Production Fungi Glucose)
