Mohy E.A., Dokhan M.A., Swelim A.M. and Amina M. Mohamed( 2015). Enhanced enzymatic saccharification of rice straw and sugarcane bagasse by Trichoderma reesei ATCC26921 cellulasesJournal of Biological Chemistry and Environmental Sciences (2015)
vol. 10 (1): 531-548.
Abstract: Rice straw (RS) and sugarcane bagasse (SCB) were subjected individually to four different distinct pretreatments: conventional alkaline (2% NaOH, 85°C for 1h), alkaline (1.5% NaOH) / H2O2, alkaline (1.5% NaOH) / NaClO and mild alkaline (1% NaOH) for 24h at room temperature. Efficiency of each pretreatment was evaluated through their effect on each lignocellulosic biomass structure as well as their susceptibility to enzymatic hydrolysis via commercial cellulases derived from Tichoderma reesei ATCC 26921. Approximately, one-half of lignin and most of hemicellulose present in both Rs and SCB were solubilized by alkaline/oxidative pretreatment at room temperature for 24h. Relative cellulose content of the pretreated samples was increased (>35%) accompanied with a decrease in crystallinity index (>2.5 times). Consequently, promotion of cellulose content and demotion of hemicellulose and lignin content facilitated the enzymatic hydrolysis (10 FPU.g-1, pH 4.8, 50°C for 24h) of treated substrate (2.5%), as it increased reducing sugars production (671.2 and 750 mg.g-1) and saccharification yield (81.54 and 87.35%) for RS and SCB, respectively. The alkaline/hypochlorite pretreatment is the most efficient method among the four tested ones.
Keywords: (enzymatic saccharification rice straw sugarcane bagasse Trichoderma reesei ATCC26921 cellulases)
